Pimeloyl-acp methyl ester
WebC, pimeloyl-ACP methyl ester is hydrolyzed by BioH to form pimeloyl-ACP, which is a substrate for BioF. D , BioF begins assembly of the biotin rings by catalyzing the pyridoxal phosphate-dependent decarboxylative … WebNov 9, 2012 · The methyl ester of this product is then cleaved by BioH to give pimeloyl-ACP which reacts with alanine in the BioF reaction to give the first intermediate of biotin ring assembly. Thus, the methyl ester disguises the biotin synthetic intermediates such that they are accepted as substrates by the fatty acid synthetic pathway [6].
Pimeloyl-acp methyl ester
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WebPimelyl- [acyl-carrier protein] methyl ester esterase activity. The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. … WebOct 8, 2012 · Once pimelate is synthesized, BioH hydrolyzes the methyl ester bond to expose the ω-carboxyl group and terminate chain elongation. The ACP moiety is subsequently released in the BioF reaction, the first …
Webpimeloyl-[acyl-carrier protein] methyl ester esterase Reaction catalysed; 6-carboxyhexanoyl-[ACP] methyl ester + H2O => 6-carboxyhexanoyl-[ACP] + H(+) + methanol: Comment(s) …
WebThe enzyme Pimelyl- [acyl-carrier protein] methyl ester esterase (EC 3.1.1.85, BioH; systematic name pimelyl- [acyl-carrier protein] methyl ester hydrolase [1] [2] [3] [4] … WebApr 6, 2024 · The pimeloyl-ACP positive control used in our assay was produced in our lab by the demethylation of pimeloyl-ACP methyl ester using the BioJ enzyme (Supplementary Fig. 7b).
WebVarious nonorthologous substitutes of the bioC -coupled gene bioH from E. coli, observed in several genomes, possibly represent the existence of different pathways for pimeloyl-CoA biosynthesis [4]. Nucleotide sequence of the bioH gene of Escherichia coli [5]. The 104-kDa protein can be converted to the 82-kDa protein upon incubation with Fe3 ...
WebPimeloyl- [acyl-carrier protein] methyl ester; Pimeloyl- [acp] methyl ester; Pimelyl- [acyl-carrier protein] methyl ester; Pimelyl- [acp] methyl ester. Formula. C8H13O3SR. Structure. … cna washington state salaryWebPimeloyl-ACP methyl ester carboxylesterase Imported Gene names ORF names SAMN05443574_103211 Imported Organism names Organism Haloarcula vallismortis (Halobacterium vallismortis) Imported Taxonomic identifier 28442 NCBI Taxonomic lineage Archaea > Euryarchaeota > Stenosarchaea group > Halobacteria > Halobacteriales > … cna washington state renewalWebFeb 12, 2016 · Helicobacter pylori lacks a gene encoding a homologue of the known pimeloyl-ACP methyl ester cleavage enzymes suggesting that it encodes a novel enzyme … ca inter direct tax study materialWebthe methyl group of the seven-carbon pimeloyl-ACP methyl ester is removed by BioH, a short-chain fatty acid esterase, to give pimeloyl-ACP, which is condensed with L-alanine by BioF to start the assembly of the fused rings of biotin (17) (Fig. 1). The E. coli protein of known function most similar to BioC cna washington licenseWebMar 9, 2024 · pimeloyl-acyl carrier protein methyl ester esterase. GeneRIFs: Gene References Into Functions. Data show a fully reversible thermal unfolding in variant forms of wild type enzyme in which a charged residue has been mutated. cna washing handsWebJul 23, 2024 · We therefore propose that the malonyl methyl ester tethered on ACP Art10 is transferred to the ACP of module 1, methylated at C-2, and then elongated ten times by modules 2 to 11 to assemble the ... ca inter eis revisionWebcomplex of BioH with pimeloyl-ACP methyl ester and use the struc-ture to demonstrate that BioH is the gatekeeper and its physio-logical substrate is pimeloyl-ACP methyl ester. cofactor biosynthesis esterase protein–protein interaction Recent work delineated the assembly pathway of the enigmatic pimeloyl moiety of biotin. cna water testing