WebPTAH stains the material which appears as electron dense deposits in electron microscopy and as granular deposits in immunofluorescence, whereas it fails to stain linear deposits … WebThe phosphotungstic acid binds all of the available hematein to form a blue lake pigment. This lake stains the muscle cross striations, fibrin, nuclei, and other tissue elements blue. …
Immunohistochemical and Ultrastructural Features of …
Phosphotungstic acid haematoxylin (PTAH) is a mix of haematoxylin with phosphotungstic acid, used in histology for staining. It stains some tissue in contrasting colors in a way similar to haematoxylin and eosin stain, as phosphotungstic acid binds to tissue proteins. It is used to show … See more There is much more phosphotungstic acid in the solution than hematein. The phosphotungstic acid binds all of the available hematein to form a blue lake pigment. This lake stains the muscle cross striations, … See more Zenker fixative is preferred, although formalin fixed tissue can be used. If this is the case, the tissue should be mordanted in Zenkers solution … See more WebJun 20, 2024 · Stain in Working Weigert's Iron Hematoxylin Solution for 5 minutes. Make Hematoxylin Solution fresh by adding equal volumes of Solution A (1% Hematoxylin in 95% EtOH) and Solution B (1.2% Ferric Chloride and 1% Acetic Acid in distilled water). The working solution is good for approximately 10 days. ** Hematoxylin stains nuclei blue-black. rock music personality
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WebMar 31, 2024 · Next, the sections were differentiated in the phosphomolybdic-phosphotungstic acid solution for 10–15 min and transferred to aniline blue solution for 5–10 min. Finally, the sections were quickly dehydrated with 95% ethanol and cleared in xylene. After drying, the sections were sealed with neutral quick-drying glue. WebPhosphotungstic Acid Hematoxylin (PTAH) Stain. Staining connective tissue - specifically collagen, striate muscle and glial fibers. ab150683. Reticulum Stain. Stains reticular fibers in connective tissue. ab150684. Safranin O Stain. Stains mucin, cartilage and mast cell granules-Steiner Stain. WebAbstract. High quality histology is needed in renal biopsy examination, especially when material for immunofluorescence and/or electron microscopy is not available. For these purposes Mallory PTAH stain was tried on 34 renal biopsies and the results were compared with the immunofluorescent and ultrastructural findings (used as a control of the ... rock music phil collins